3rd lab - determination of glucose content using DNS method

well, this time I'm too tooo tooooo lazy to take pics of the kids, and hell..those kids really know how to mess things up..the lab was done in 2 sessions (which means, 2 weeks consecutively) and then the eid hols comes in..dem..im lazy to talk..coz im actually waiting for the materials to be autoclaved.. (around 1hr more to finish).. y am i still at lab at 8.30pm of saturday ni? even to myself, it is still a wonder.


the concepts behind the sugar measurements was developed due to the solutions having no colour or odour whatsoever. hence detection was difficult. but that was WAY WAY WAY back then. this method is the cheapest (as in most economical), quite laborous, abit hazardous but rather precise in detecting total amount of simple sugar..which has been established a looooog time ago..what is simple sugar? simple sugar is the basic unit of carbs (i think so..pardon me if im wrong, i juz pour out frm my brain), which has 3 elements, C, H and O..aiya..carbon, hydrogen and oxygen atom..with the formation of C6H12O6..with the carbon acting as a skeleton..an extended shape has hundreds and thousands of C in them with different branchings..ehh..mcm melalut..

back to main topic..so basically, by having a standard curve that has been produced earlier with a known amount of gluose used, one can determine through the calculated amount of light that can pass through a certain solution..the darker the colour, the harder for light to go through kan? this is known as transmittant light at which reciprocating it will result in the calculation of absorbance.. (absorbance in this case means, how much light has been absorbed by the sample which can be detected by a spectrophotometer at UV-vis range)

so basically, its an easy lab..but the hardest part was to make the standard..u need to be super precise..if not, all ur work is a goner lah..

aigooo..lapa

ade keje nak wat lg tapi mls..

agar well xtest lg..

aigoo..

aigooooo... T_____________T|||||




*sigh*